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@article{185700,
        author = {HUSMIN RISHA T and Dr. A. L. Hema Latha},
        title = {Optimization of Lipase Production by Enterobacter ludwigii isolated from Fish Processing Industry Waste Soil},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {12},
        number = {5},
        pages = {2688-2693},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=185700},
        abstract = {Lipases are versatile enzymes with applications in detergents, biodiesel production, and the food industry. In this study, a lipase-producing bacterium was isolated from fish processing industry waste soil using Phenol Red Olive Oil Agar. The most potent isolate was identified as Enterobacter ludwigii by morphological, biochemical, and 16S rRNA sequence analysis (GenBank Accession No. PX112880, 99.7% similarity). Lipase production was optimized using the One-Factor-At-A-Time (OFAT) method. Maximum lipase activity (160.2 U/mL) was obtained at pH 8.0, 37°C, 1% glucose, 0.5% peptone, 1% olive oil, and 48 h incubation. Partial purification resulted in a 1.8-fold increase in specific activity, with optimal activity at pH 8.0 and 45°C. The enzyme retained 82% activity after 1 h at 40°C. These results suggest that E. ludwigii from fish waste soil is a potent lipase producer suitable for industrial applications.},
        keywords = {Lipase, Enterobacter ludwigii, Phenol Red Agar, Fish waste soil, OFAT optimization, 16S rRNA},
        month = {October},
        }