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@article{186465,
        author = {Tania Shroff and Pranali Joshi and Purva Patil and Dr. Geetanjali Ganguli},
        title = {Unveiling the role of phytochemicals in abating Antimicrobial Resistance in Staphylococcus aureus},
        journal = {International Journal of Innovative Research in Technology},
        year = {},
        volume = {12},
        number = {no},
        pages = {172-181},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=186465},
        abstract = {On a global scale antimicrobial resistance (AMR) represents a significant menace to public health. Staphylococcus aureus is a member of the ESKAPE pathogens group which are of special concern owing to the rapid development of resistance. This has been correlated with a few mechanisms, one such being the expression of efflux pumps on the cell surface. The efflux of Antibiotics via bacterial MDR efflux pumps contributes to increased AMR prevalence heightening the need to counter this resistance mechanism by the discovery of novel therapeutics like efflux pump inhibitors (EPIs) that target the MDR (Multiple Drug Resistance) efflux pumps that are overexpressed to reduce antibiotic efficacy. Natural phytomolecules such as Piperine (PIP) an alkaloid found in Piperaceae family and Limonene (LMN) a terpene found in Rutaceae family show promise as novel natural EPIs by potentiating the activity of antibiotics. The present work aims to demonstrate the synergy and increase in efficacy (inhibition) of Tetracycline when coupled with these phytomolecules at their therapeutic concentrations. Further through an Ethidium Bromide Efflux Assay the role of these phytomolecules as EPI’s is also explored. A significant fold reduction in bacterial viability was observed indicating that phytomolecules potentiate the effect of the antibiotic. In silico docking further validated that the phytochemical piperine, demonstrated higher binding affinity with some known efflux pumps, such as NorA and ABC transporter proteins. The study indicates that augmented inhibition can be achieved at the same dosage or lower, translating to the use of lower therapeutic concentrations of antibiotics for treatments of these infections when supplemented with phytomolecules, a crucial step in combating the rise of AMR.},
        keywords = {AMR, Efflux Pump Inhibitors, Phytomolecules, Staphylococcus aureus},
        month = {},
        }