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@article{186483,
        author = {R. Dalvi and V. Pardeshi and S. Singh and R. Kadam and S. Surve},
        title = {Assessment of a Polycomponent Biostimulant for Improving Growth, Yield, and Salinity Tolerance in Spinach (Spinacia oleracea)},
        journal = {International Journal of Innovative Research in Technology},
        year = {},
        volume = {12},
        number = {no},
        pages = {274-281},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=186483},
        abstract = {Salinity stress is a major abiotic factor limiting agricultural productivity, necessitating eco-friendly strategies to enhance crop resilience. This study evaluates the effectiveness of microbial and polycomponent biostimulants in alleviating salt stress in spinach (Spinacia oleracea). Soil-derived Bacillus subtilis and freshwater algae (Chlorella sp. and Diatoms) were isolated and morphologically characterized as potential biostimulants. Their monocomponent, dual-component, and polycomponent formulations were assessed along a gradient of NaCl concentrations ranging from 25 to 100 mM for their effects on seed germination, plantlet growth, and physiological performance.
Among monocomponent treatments, algae exhibited the highest growth-promoting activity under saline conditions. In dual-component combinations, Proline + Humic Acid outperformed Algae + Bacteria, at higher salinity levels. Notably, the polycomponent biostimulant formulation Poly6—comprising 0.01% each of humic acid, proline, algae, and bacteria—demonstrated the highest efficacy. Poly6 promoted superior seed germination, plantlet survival, and overall biomass accumulation under both normal and saline soil conditions.
Biochemical analysis revealed that Poly6-treated plants exhibited elevated total chlorophyll content (1.09 mg/g) and the highest antioxidant activity (Radical Scavenging Activity: 89.72%), indicating enhanced photosynthetic capacity and improved oxidative stress tolerance. These findings suggest that polycomponent biostimulants Poly6, offer a promising and sustainable approach for improving salinity stress. Further field validation and mechanistic studies are recommended to optimize its application in diverse agricultural systems.},
        keywords = {Abiotic stress tolerance, Biostimulants, Bacillus subtilis, Chlorella, Humic acid, Proline, Polycomponent formulations, RSA (Radical Scavenging Activity), Sustainable agriculture, Soil salinity},
        month = {},
        }