Copyright © 2026 Authors retain the copyright of this article. This article is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

@article{187627,
        author = {Dr.T.Yamini kumari and K.Keerthi and K.Girish and J.Saritha},
        title = {Review Article on Hyphenated Techniques and Their Inter Face in Pharmaceutical Analysis},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {12},
        number = {6},
        pages = {5972-5982},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=187627},
        abstract = {The combination or coupling of the various analytical approaches is known as the hyphenated technique. Spectroscopic methods are generally paired with chromatographic methods. After that, an interphase will allow the mixture's separated components from the chromatographic process to enter the spectroscopic method. The separated components from gas chromatography are fed into the mass spectrometer (MS) for ionization, mass analysis, and the detection of mass-to-charge ratios of ions produced by each analysis. GC and MS can be connected using a jet/orifice separator, effusion separator, or membrane separator. The analytical flow cell was first built for continuous flow to NMR in LCNMR coupling. However, the use of the stopped-flow mode in LCMS has resulted from the necessity for a thorough structural evaluation of novel natural compounds. The use of LC-MS-MS is rapidly growing every day. When combined with biological screening, hyphenated techniques like HPLC coupled to UV and mass spectrometry (LC-UV-MS) have proven to be very helpful for a quick survey of natural compounds. These days, there are many distinct kinds of LC-MS systems with diverse interfaces that are sold commercially. Hyphenated procedures are used to describe separation and identification, and they provide improved sample analysis by incorporating specificity, accuracy, and precision.},
        keywords = {Hyphenated Techniques, Interphase and Benefits},
        month = {November},
        }