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@article{191767,
        author = {I VEENA RANI and HANMAKONDA VIJAYA LAXMI},
        title = {CROCUS SATIVUS METHANOL EXTRACTS IMMUNOMODULATORY PROPERTIES IN WISTAR RATS IN ALBINO SWISS MICE},
        journal = {International Journal of Innovative Research in Technology},
        year = {2026},
        volume = {12},
        number = {8},
        pages = {8364-8372},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=191767},
        abstract = {Background: Crocus sativus (saffron) is a medicinal plant known for its diverse pharmacological properties, including antioxidant, anti-inflammatory, and immunomodulatory effects. This study aimed to evaluate the immunomodulatory potential of Crocus sativus methanolic extract on both cell-mediated and humoral immune responses in experimental animal models.
Methods
The extract was administered orally at doses of 200 mg/kg and 400 mg/kg daily for 14 days. Four groups were included: control (vehicle), two treatment groups receiving Crocus sativus extract, and a standard group treated with Levamisole (50 mg/kg). Delayed Type Hypersensitivity (DTH) response was assessed as a measure of cell-mediated immunity, while Humoral Antibody (HA) titers were measured to evaluate humoral immune response. Total leukocyte count (TLC) and differential leukocyte count (DLC) were also determined.
Results
Crocus sativus extract significantly enhanced the DTH response in a dose-dependent manner, comparable to Levamisole at the higher dose. Both primary and secondary HA titers were markedly increased in treated groups, indicating stimulation of humoral immunity. TLC showed a significant increase after treatment, along with a higher percentage of lymphocytes and reduced neutrophil counts, suggesting modulation of immune cell populations. These effects demonstrate the immunostimulatory potential of Crocus sativus.
Conclusion
The methanolic extract of Crocus sativus enhances both cell-mediated and humoral immune responses, increases leukocyte proliferation, and modulates leukocyte distribution. These findings support its potential use as a natural immunostimulant and warrant further investigation for therapeutic applications.},
        keywords = {DTH response, Crocus sativus, immunomodulatory, leukocyte proliferation},
        month = {January},
        }