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@article{210912,
        author = {Jyoti Patel and Raj Kumar},
        title = {Rational Design and Multi-Marker HPTLC Standardization of Polyherbal Antioxidant Formulations: A Review},
        journal = {International Journal of Innovative Research in Technology},
        year = {2026},
        volume = {13},
        number = {6},
        pages = {2714-2724},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=210912},
        abstract = {Polyherbal antioxidant formulations are widely used in traditional and complementary medicine, yet their quality control is frequently limited to pharmacognostic and single-marker analysis, an approach insufficient for the complex, multi-herb matrices such products actually are. This review examines the rational, design-of-experiments-guided development and chemical standardization of a five-herb polyherbal antioxidant formulation comprising Phyllanthus emblica (Amla), Curcuma longa (Turmeric), Camellia sinensis (Green tea), Ginkgo biloba and Ocimum sanctum (Tulsi), optimised by a simplex-lattice mixture design for maximal phenolic and flavonoid content and free-radical-scavenging activity. The phytochemical basis, detection chemistry and structure-antioxidant-activity relationships of six representative markers, gallic acid, ascorbic acid, curcumin, catechin, quercetin and eugenol, are discussed alongside the validated high-performance thin-layer chromatography (HPTLC) fingerprinting methodology proposed for their simultaneous multi-marker quantification, the in-vitro antioxidant assay battery (DPPH, ABTS, FRAP, total phenolic and flavonoid content) against which the formulation is to be benchmarked, and the statistical framework proposed for correlating marker content with measured antioxidant activity. Precedent from twenty-nine directly relevant chromatographic, phytochemical and analytical-validation studies is synthesised to situate the proposed formulation within the wider marker-based standardization literature, and the regulatory basis for validating the resulting method is set out.},
        keywords = {Polyherbal formulation; HPTLC fingerprinting; multi-marker quantification; antioxidant activity; simplex-lattice mixture design; phytochemical standardization; DPPH; quercetin; curcumin; gallic acid},
        month = {November},
        }