Quantitative Determination of Antibacterial Susceptibility Using BD Phoenix for the Clinical Isolates from IBD Patients and Inhibition Against Biological Chitosan using Resazurin Assay
V.Mythili, O.S. Aysha
Chitosan is a bio polymer which has multiple pharmacological units. Chitosan can also be used to treat various types of infections and diseases. One amongst is ulcerative colitis. The biological chitosan was synthesised using mushroom and the soil isolate Bacillus spp. The production media was prepared and the chitosan was synthesized. The clinical isolates were isolated from IBD stool samples such as E.coli, K.pnuemoniae, P.aeruginosa and S. aureus. The antibacterial activity of biological chitosan against E. coli, K.pnuemoniae, P.aeruginosa and S. aureus was evaluated by calculation of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results show that 90 % of the drugs were sensitive to most of the isolates among the analysed 20 drugs. The resistant drug was nalidixic acid to E. coli whereas ampicillin was resistant to K.pneumoniae. Gentamicin and tigecycline were resistant to P. aeruginosa and rifampicin and trimethoprim were found resistant to S. aureus. A visual reading of the direct REMA, which was the best method for distinguishing resistant and susceptible strains, indicated that the MIC cut-off points for the biological chitosan was 3200 µg/ml.
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Unique Paper ID: 150675

Publication Volume & Issue: Volume 7, Issue 9

Page(s): 9 - 14
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