Pramod S. Dalal, Sangram U. Deshmukh, Nandkishor B. Bavage, Shyamlila B. Bavage
In the pharmaceutical industry, all manufactured products need to be of the highest quality to ensure the least risk to patients. To guarantee that goods pass certain standards, researchers, manufacturers and developers use various technical equipment and analytical techniques, including liquid chromatography, during the development process. Liquid chromatography is an analytical technique that is used to separate a certain sample into its individual components. The separation occurs when the sample interacts with the mobile (liquid) and stationary phases (column). The various parts of the sample are separated out based on their polarities; they will have varying levels of affinity for the mobile phase, resulting in migration through the column at different speeds. The mixed components are placed at the top of the column of the stationary phase, which is generally a fine adsorbent solid such as silica. This must be distributed evenly to minimise the presence of air bubbles that could influence the results of the test. The exit of the column is stoppered with glass, wool or a porous plate. When the mobile phase passes through, the mixture separates into bands. These can then be collected and analysed via other methods. The technique works as the components in a mixture are attracted to the adsorbent surface of the stationary phase with varying degrees depending on their individual polarity and their unique structural characteristics; a component with a higher affinity for the stationary phase will migrate down the column slower than a component which has more affinity for the mobile phase. The most common form of liquid chromatography in use today is high-performance liquid chromatography (HPLC), which pumps the sample mixture through the column at high pressure.
Article Details
Unique Paper ID: 152147

Publication Volume & Issue: Volume 8, Issue 2

Page(s): 506 - 511
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