USE OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY IN THE PHARMACEUTICAL INDUSTRY

Pramod S. Dalal; Sangram U. Deshmukh; Nandkishor B. Bavage; Shyamlila B. Bavage

USE OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY IN THE PHARMACEUTICAL INDUSTRY

Authors: Pramod S. Dalal, Sangram U. Deshmukh, Nandkishor B. Bavage, Shyamlila B. Bavage

Unique Paper ID: 152147
Volume: 8
Issue: 2
PageNo: 506-511

Keywords: No Keywords Found

Abstract:
In the pharmaceutical industry, all manufactured products need to be of the highest quality to ensure the least risk to patients. To guarantee that goods pass certain standards, researchers, manufacturers and developers use various technical equipment and analytical techniques, including liquid chromatography, during the development process. Liquid chromatography is an analytical technique that is used to separate a certain sample into its individual components. The separation occurs when the sample interacts with the mobile (liquid) and stationary phases (column). The various parts of the sample are separated out based on their polarities; they will have varying levels of affinity for the mobile phase, resulting in migration through the column at different speeds. The mixed components are placed at the top of the column of the stationary phase, which is generally a fine adsorbent solid such as silica. This must be distributed evenly to minimise the presence of air bubbles that could influence the results of the test. The exit of the column is stoppered with glass, wool or a porous plate. When the mobile phase passes through, the mixture separates into bands. These can then be collected and analysed via other methods. The technique works as the components in a mixture are attracted to the adsorbent surface of the stationary phase with varying degrees depending on their individual polarity and their unique structural characteristics; a component with a higher affinity for the stationary phase will migrate down the column slower than a component which has more affinity for the mobile phase. The most common form of liquid chromatography in use today is high-performance liquid chromatography (HPLC), which pumps the sample mixture through the column at high pressure.

Download article

email to a friend

Cite This Article

ISSN: 2349-6002
Volume: 8
Issue: 2
PageNo: 506-511

USE OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY IN THE PHARMACEUTICAL INDUSTRY

Available:https://ijirt.org/Article?manuscript=152147

11 Jan, 2022

EVALUATION OF MARKETED EFFERVESCENT TABLETS

13 Oct, 2021

Bacterial Biofilm Inhibitor

02 Aug, 2021

The risk of death caused by the use of norepinephrine-resistant hypotension in the condition of septic shock in a patient

02 Aug, 2021

COMPUTER AIDED DRUG DESIGN (CADD)

29 Jul, 2021

Impact : Of Second Wave Covid-19 In world

29 Jul, 2021

The use of milrinone for curing a chronic heart failure

26 Jul, 2021

Leprosy Disease review

20 Jul, 2021

Transdermal Drug Delivery System

13 Jul, 2021

IBUPROFEN: AN OVERVIEW

13 Jul, 2021

CHEMOTHERAPY FOR BREAST CANCER

Impact Factor
8.01 (Year 2024)

UGC Approved
Journal no 47859

Join Our IPN

IJIRT Partner Network

Submit your research paper and those of your network (friends, colleagues, or peers) through your IPN account, and receive 800 INR for each paper that gets published.

Join Now

Latest Publication

Recent Conferences

NCSEM 2024

National Conference on Sustainable Engineering and Management - 2024 Last Date: 15th March 2024

Submit inquiry