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@article{181652,
        author = {Krushna Raghu Bharwad and Hole Pooja Jalindar and Janhavi Navin Patil and Sakshi Ganesh  Bhandekar and Amruta Kailas Bhangarath and Sneha Maroti Chitkalwar and Sagar Sundar Edke and Rahul Balaji Malode},
        title = {A BLOTTING TECHNIQUES TO DETECTIONS AND IDENTIFICATION OF BIOMOLECULES},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {12},
        number = {1},
        pages = {4810-4819},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=181652},
        abstract = {Biological macromolecules constitute large molecules essential for life that are constructed from smaller organic molecules. The four primary categories of biological macromolecules include carbohydrates, lipids, proteins, and nucleic acids (DNA and RNA). Carbohydrates derive from monosaccharides, lipids from fatty acids, proteins from amino acids, and nucleic acids from nucleotides.In molecular biology laboratories, scientists often need to isolate specific molecules from particular macromolecules such as protein, DNA, or RNA. This process is termed "Blotting," a methodology whereby macromolecules like DNA, RNA, or proteins are separated in a gel matrix, subsequently transferred to a solid support, and detected using a specific probe.
Blotting techniques enable researchers to identify and characterize specific molecules within complex mixtures through a three-step procedure: initial separation via gel electrophoresis, transfer of the gel onto a membrane/paper, and visualization of the target molecule using a specific visualizing agent.
The nomenclature of blotting varies according to the target molecule: protein analysis is designated "Western Blotting" or "Immunoblotting," DNA analysis is termed "Southern Blotting," and RNA analysis is referred to as "Northern Blotting.”},
        keywords = {Blotting,DNA, Electrophoresis, Northern Blotting, Protein,Probe,RNA,Western Blotting,Southern Blotting, Western Blotting,},
        month = {June},
        }