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@article{190248,
        author = {Kathi Amulya and Syeda Nishat Fathima},
        title = {ETHNOPHARMACOLOGICAL VALIDATION OF BARLERIA TERMINALIS NEES: COMPARATIVE IN VITRO ANTI-INFLAMMATORY EVALUATION OF LEAVES, STEMS AND FLOWER},
        journal = {International Journal of Innovative Research in Technology},
        year = {2026},
        volume = {12},
        number = {8},
        pages = {590-595},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=190248},
        abstract = {Ethnopharmacological relevance: Barleria terminalis Nees (Acanthaceae) is traditionally used in Indian folk medicine for the treatment of inflammatory conditions, wounds, pain, and fever. However, experimental evidence supporting its anti-inflammatory potential, particularly across different plant parts, remains limited.
Aim of the study: The present study aimed to evaluate the in vitro anti-inflammatory activity of aqueous and ethanolic extracts of leaves, stems, and flowers of Barleria terminalis.
Materials and methods: Extracts of leaves, stems, and flowers were prepared using aqueous and ethanolic solvents and evaluated for in vitro anti-inflammatory activity using inhibition of protein denaturation, heat-induced haemolysis, human red blood cell (HRBC) membrane stabilization, and proteinase inhibitory assays. Diclofenac sodium was used as the reference anti-inflammatory drug. All experiments were performed in triplicate, and results were expressed as mean ± SD.
Results: All extracts exhibited significant, concentration-dependent anti-inflammatory activity across the tested models. Ethanolic extracts showed consistently higher activity than aqueous extracts. Among the different plant parts, leaf extracts demonstrated the strongest anti-inflammatory effects, followed by flower and stem extracts. Ethanolic leaf extract showed pronounced inhibition of protein denaturation, effective stabilization of erythrocyte membranes, and notable proteinase inhibitory activity, with effects approaching those of diclofenac sodium at higher concentrations.
Conclusion: The findings provide experimental support for the traditional use of Barleria terminalis in inflammatory disorders. The observed in vitro anti-inflammatory activity, particularly of leaf extracts, suggests that the plant is a promising source of bioactive compounds warranting further phytochemical characterization and in vivo validation.},
        keywords = {Barleria terminalis; Anti-inflammatory activity; Protein denaturation; HRBC membrane stabilization},
        month = {January},
        }