Antibacterial Activity Of Vacuum Liquid Chromatography (Vlc) Isolated Fractions Of Chloroform Extracts Of Seeds Of Achyranthes Aspera
Author(s):
Biradar Sachin, Renuka R. Deshpande, Nandkishor B. Bavage, Vidyasagar Gali, Shyamlila B. Bavage
Keywords:
Achyranthes aspera, Vacuum liquid chromatography, Isolated fractions, Antibacterial activity.
Abstract
Antibacterial activities of locally occurring weed Achyranthes aspera were studied.Three solvents (Hexane, Chloroform, and Ethanol) were used successively for the extraction ofactive principles from the seeds of this plant. The extracts were concentrated on vacuum rotaryevaporator. The concentrated extracts were tested for their antibacterial activities after making theirsolution in gum acacia. The six bacterial strains used in the antibacterial studies were Bacillus subtilis Micrococcus luteus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeuroginosa and Salmonella chloerasuis. Antibacterial activities of the extracts were compared with streptomycinand ampicillin in terms of zones of inhibition. Chloroform and ethanol extracts demonstratedantibacterial activity. Hexane extract did not demonstrate antibacterial activity. Chloroform extractwas more potent than alcohol extract in terms of antibacterial activity. An attempt was made toidentify the nature of compound by isolation through vacuum liquid chromatography (VLC). Thefractions isolated by VLC were subjected to thin layer chromatography (TLC). TLC showed the presence of alkaloids and terpenoids. The active fractions were tested for their antibacterial activity.One of the fractions exhibited antibacterial activity.
Article Details
Unique Paper ID: 149468

Publication Volume & Issue: Volume 6, Issue 12

Page(s): 454 - 458
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Last Date 25 August 2020

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