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@article{208322,
        author = {Dr Asha Meena and Dr Gyan Prakash Sharma and Dr Meenakshi Sharma and Dr Gouri Shankar Rajpurohit and Dr Tarun Meena},
        title = {HPTLC Fingerprinting and Standardization of Asthishrinkhala (Cissus quadrangularis Linn.) Raw Powder and Vati Formulation: An Analytical Quality Control Study},
        journal = {International Journal of Innovative Research in Technology},
        year = {2026},
        volume = {13},
        number = {4},
        pages = {1578-1585},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=208322},
        abstract = {Asthishrinkhala (Cissus quadrangularis Linn.) is a highly revered medicinal plant in Ayurveda, renowned for its bone healing properties. The presence of diverse phytoconstituents, including triterpenoids, flavonoids, steroids, and phenolic compounds, necessitates robust quality control measures to ensure batch to batch consistency and therapeutic efficacy in its finished formulations. Objective: To establish a reproducible High Performance Thin Layer Chromatography (HPTLC) fingerprint profile for Asthishrinkhala raw powder and its Vati (tablet) formulation, identifying characteristic biomarker constituents. Methods: Samples of raw powder (RA) and Vati formulation (SA) were extracted in methanol and applied onto Merck TLC Silica gel 60 F254 plates. Chromatographic separation was achieved using a mobile phase of formic acid:ethyl acetate:toluene (1:4:5 v/v/v) under saturated conditions (20 minutes). Detection and documentation were performed at 254 nm, 366 nm, and under white light using a CAMAG TLC Visualizer 3. Results: Under UV 254 nm, a distinct band at Rf 0.38 was observed in all tracks. Under UV 366 nm, three prominent fluorescent bands were detected at Rf 0.55, 0.73, and 0.83. The band at Rf 0.38 correlated with quercetin, and the band at Rf 0.55 correlated with B sitosterol, based on reported reference data for Cissus quadrangularis. The fingerprint profiles of the raw powder and the Vati formulation were identical across all applied volumes (2.0-8.0 uL), demonstrating consistent phytochemical composition. Conclusion: The developed HPTLC method is reliable, reproducible, and suitable for the identification and standardization of Asthishrinkhala raw materials and formulations. The identical profiles confirm the efficient preservation of key biomarkers during the manufacturing process, supporting the quality assurance framework for Asthishrinkhala based products.},
        keywords = {Asthishrinkhala, Cissus quadrangularis, HPTLC fingerprinting, quercetin, B-sitosterol, quality control, Vati},
        month = {September},
        }