Phytochemical Screening and Determination of Antibacterial Activities of Silver Nanoparticles of White Button Mushroom (Agaricus bisporus)
Keshamma E., Suresh Kumar C., Manjula A.C., Prathibha K.Y.
Agaricus bisporus, AgNPs, Phytochemical Screening, Antibacterial Activity.
Present study was carried out with the main purpose of phytochemical screening of edible mushroom Agaricus bisporus, synthesis of silver nanoparticles of aqueous (aq.) extract of A. bisporus and to evaluate their antibacterial potential. The crude extract A. bisporus was prepared by maceration method by boiling continuously for 30 minutes in water bath. The silver nanoparticle (AgNPs) of A. bisporus was prepared by mixing 1ml of aq. extract of A. bisporus and 9 ml of 1mm stock solution of AgNOꝫ. The antibacterial activity assay was carried out by agar well diffusion method. Results revealed that the phytochemical analysis of aq. extract of Agaricus bisporus disclosed the presence of secondary metabolites viz. terpenoids, saponins, steroids, alkaloids, phenols, insulin and glycosides. Antibacterial activity assay delineated that aqueous extract of A. bisporus was effective against S. aureus and B. amyloliquefacien. Furthermore, antibacterial activity assay results depicted the highest efficacy of AgNPs of A. bisporus as compared to aq. extract of A. bisporus alone. In conclusion, AgNPs of A. bisporus could be recommended to use for biomedical and pharmaceutical applications.
Article Details
Unique Paper ID: 152819

Publication Volume & Issue: Volume 8, Issue 4

Page(s): 490 - 495
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