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@article{187349,
        author = {Miss. Saundarya D. Rajput and Miss. Pranjali S. Suryawanshi and Miss. Diksha Narwade},
        title = {Development of a Herbal Mouthwash containing Mimosa pudica and Casein for Tannin Reduction},
        journal = {International Journal of Innovative Research in Technology},
        year = {2025},
        volume = {12},
        number = {6},
        pages = {3918-3925},
        issn = {2349-6002},
        url = {https://ijirt.org/article?manuscript=187349},
        abstract = {Herbal mouthwashes are increasingly being recognized as safe and effective alternatives to conventional chemical-based oral care products due to their biocompatibility, minimal side effects, and natural therapeutic potential. This review focuses on the development of a novel herbal mouthwash containing Mimosa pudica and casein aimed at reducing tannin-induced staining and promoting overall oral health. Mimosa pudica, widely known for its antimicrobial, antioxidant, and anti-inflammatory properties, can help control oral microbial growth and oxidative stress, while casein, a milk-derived phosphoprotein, exhibits a high affinity for polyphenols and tannins, facilitating their precipitation and removal from the oral cavity. The formulation process typically involves the aqueous extraction of Mimosa pudica leaves using maceration or Soxhlet techniques, followed by filtration and concentration of the extract. Casein is then incorporated into the extract along with excipients such as humectants, preservatives, and flavoring agents to achieve a stable, palatable, and safe mouthwash. The final product is subjected to rigorous evaluation, including physicochemical parameters such as pH, viscosity, and organoleptic characteristics (taste, color, and odor), as well as stability studies under different storage conditions. Functional assessment involves measuring tannin-binding efficacy, antimicrobial activity against common oral pathogens, and cytotoxicity assays to ensure biocompatibility. The integration of plant bioactives with protein-based agents like casein provides a synergistic approach to reduce tannin levels, enhance oral hygiene, and offer a natural alternative to chemical mouthwashes. This review highlights the formulation strategies, mechanisms of tannin reduction, and evaluation parameters critical for developing effective herbal oral care products, demonstrating the potential of Mimosa pudica and casein-based mouthwashes as safe, natural, and functional oral hygiene solutions.},
        keywords = {Mimosa pudica, casein, herbal mouthwash, tannin reduction, oral health, formulation, natural oral care.},
        month = {November},
        }